Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Review of percutaneous therapy for bifurcation lesions in the era of drug-eluting stents.

Minerva cardioangiologica·2008

Same author

Relationship between heat shock protein 60 (HSP60) mRNA expression and resistance to platinum analogues in human ovarian and bladder carcinoma cell lines.

Cancer letters·2008

Same author

Expression of genes related to activity of oxaliplatin and 5-fluorouracil in endoscopic biopsies of primary esophageal cancer in patients receiving oxaliplatin, 5-flourouracil and radiation: characterization and exploratory analysis with survival.

Journal of chemotherapy (Florence, Italy)·2006

Same author

S-phase modulation by irinotecan: pilot studies in advanced solid tumors.

Cancer chemotherapy and pharmacology·2005

Same author

Ubiquinone does not rescue acute myeloid leukemia cells from growth inhibition by statins.

Leukemia·2003

Same author

In vitro studies on the mechanisms of oxaliplatin resistance.

Cancer chemotherapy and pharmacology·2002

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Published on: May 28, 2020

RT-PCR Quantitation of HSP60 mRNA Expression.

R P Perez¹, L Pendyala, Z Elakawi

¹Section of Hematology/Oncology, Department of Medicine, Darthmouth-Hitchcock Medical Center, Lebanon, NH.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Heat shock protein 60 (HSP60) aids protein folding and cellular protection. Its gene expression is linked to cisplatin resistance in ovarian and bladder cancers.

More Related Videos

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Published on: January 3, 2025

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Related Experiment Videos

Last Updated: Jun 4, 2026

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Published on: May 28, 2020

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris

Published on: January 3, 2025

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

Heat shock protein 60 (HSP60) is a chaperonin crucial for protein folding, assembly, and transport within mitochondria.
HSP60 protects cellular proteins from denaturation during heat stress.
The HSP60 gene (HSPD1) is highly conserved and its sequence is well-documented.

Purpose of the Study:

To investigate the role of HSP60 in cancer, specifically its association with cisplatin resistance.
To quantify HSP60 mRNA expression in preclinical models of human ovarian and bladder carcinomas.

Main Methods:

Utilized preclinical human ovarian and bladder carcinoma models.
Quantitated messenger RNA (mRNA) expression levels of HSP60.

Main Results:

HSP60 gene expression was quantified in preclinical human ovarian and bladder carcinoma models.
Previous studies indicate a correlation between HSP60 expression and cisplatin resistance.

Conclusions:

HSP60 plays a significant role in cellular processes, particularly under stress conditions.
Further research into HSP60's role may elucidate mechanisms of cisplatin resistance in ovarian and bladder cancers.