Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wildfire-related smoke inhalation worsens cardiovascular risk in sleep disrupted rats.

Frontiers in environmental health·2023
Same author

A checklist of larval Digenea (Platyhelminthes: Trematoda) in molluscs from inland waters of Argentina: one hundred years of research.

Journal of helminthology·2022
Same author

Influence of environmental variability and Emiliania huxleyi ecotypes on alkenone-derived temperature reconstructions in the subantarctic Southern Ocean.

The Science of the total environment·2021
Same author

Optical pupillometry in traumatic brain injury: neurological pupil index and its relationship with intracranial pressure through significant event analysis.

Brain injury·2019
Same author

In our opinion.

British dental journal·2017
Same author

High-dose chemotherapy and autologous stem cell transplantation for primary central nervous system lymphoma: a multi-centre retrospective analysis from the United Kingdom.

Bone marrow transplantation·2017
Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015
Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014
Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014
Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014
Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014
Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Cell separations by flow cytometry.

D Davies1

  • 1FACS Laboratory, Imperial Cancer Research Fund, London, UK.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Flow cytometry analyzes cell characteristics using lasers and detectors. This technology quantifies physical and chemical properties of cells in a fluid stream, offering objective and precise measurements.

More Related Videos

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Related Experiment Videos

Last Updated: Jun 4, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
09:39

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Flow cytometry is a technique for analyzing physical and chemical properties of particles in a fluid stream.
  • Modern flow cytometers utilize a light source (laser), optics, detectors, and computer for data analysis.
  • It functions as a quantitative fluorescence microscope, replacing human observation with objective instrumentation.

Purpose of the Study:

  • To describe the fundamental principles and components of flow cytometry.
  • To explain how flow cytometers measure cellular characteristics.
  • To highlight the quantitative and objective nature of flow cytometry.

Main Methods:

  • Cells in a fluid stream pass through a laser beam.
  • Light scattering and fluorescence emission from fluorochromes are measured.
  • Optical filters and detectors isolate and quantify specific wavelengths for up to six fluorochromes.

Main Results:

  • Flow cytometry provides objective and quantitative data on cellular properties.
  • The technique allows for the measurement of multiple fluorochromes simultaneously.
  • Detailed optical setups enable precise analysis of scattered and emitted light.

Conclusions:

  • Flow cytometry is a powerful tool for detailed particle analysis.
  • The technology enhances objectivity and quantitation in biological measurements.
  • Understanding flow cytometry principles is crucial for its effective application.