Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

Cell aggregation assays.

T Boterberg¹, M E Bracke, E A Bruyneel

¹Laboratory of Experimental Cancerology, Department of Radiotherapy, Nuclear Medicine and Experimental Cancerology, University Hospital Gent, Gent, Belgium.

Methods in Molecular Medicine

|February 23, 2011

Summary

Carcinoma cell invasion occurs due to an imbalance in invasion-regulating genes. The E-cadherin/catenin complex, crucial for cell adhesion, is often downregulated in invasive cancers, impacting cell behavior.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Patterns and quality of therapeutic care for glioma: a population-based study in Belgium between 2016 and 2019.

ESMO open·2026

Same author

Temporal muscle measurements as predictor for outcome in a cohort of IDH-wildtype glioblastoma patients.

Brain & spine·2026

Same author

Sex-based survival differences in IDH-wildtype glioblastoma: Results from a retrospective cohort study.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2021

Same author

Central nervous system relapse in high-risk stage 4 neuroblastoma: The HR-NBL1/SIOPEN trial experience.

European journal of cancer (Oxford, England : 1990)·2020

Same author

Subventricular zone contacting glioblastoma: tumor size, molecular biological factors and patient survival.

Acta oncologica (Stockholm, Sweden)·2020

Same author

Weak MGMT gene promoter methylation confers a clinically significant survival benefit in patients with newly diagnosed glioblastoma: a retrospective cohort study.

Journal of neuro-oncology·2019

Area of Science:

Cell Biology
Molecular Oncology
Cancer Research

Background:

Carcinoma cell invasion is driven by an imbalance between invasion promoter and suppressor genes.
The E-cadherin/catenin complex, a key invasion suppressor in epithelioid cells, links cell membranes to the actin cytoskeleton.
Downregulation of this complex is frequently observed in invasive carcinomas, stemming from genetic or functional defects.

Purpose of the Study:

To investigate the role of the E-cadherin/catenin complex in carcinoma cell invasion.
To explore the functional integrity of the E-cadherin/catenin complex in epithelioid tumor cells.

Main Methods:

Utilizing cell aggregation assays to assess the functionality of the E-cadherin/catenin complex.
Comparing cell aggregation in vitro between invasive and noninvasive cell types.

More Related Videos

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

Related Experiment Videos

Last Updated: Jun 4, 2026

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

Main Results:

Functional integrity of the E-cadherin/catenin complex is essential for cell-cell adhesion in epithelial cells.
Cell aggregation assays effectively differentiate between invasive and noninvasive cell behaviors.

Conclusions:

The E-cadherin/catenin complex plays a critical role in suppressing carcinoma cell invasion.
In vitro cell aggregation assays are valuable tools for studying invasion mechanisms in cancer.