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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jun 4, 2026

AQRNA-seq for Quantifying Small RNAs
05:12

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Effects of collection, processing, and storage on RNA detection and quantification.

M Holodniy1

  • 1AIDS Research Center, Veterans Affairs Health Care System, Palo Alto, CA.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary

Molecular diagnostics enable direct detection of infectious agents, overcoming limitations of traditional culture methods for unculturable pathogens like certain viruses. This advances disease evaluation and therapeutic response monitoring.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Area of Science:

  • Clinical diagnostics
  • Microbiology
  • Molecular biology

Background:

  • Traditional disease evaluation relies on clinical endpoints, surrogate markers, and microbiologic culture methods.
  • Standard culture techniques are effective for many bacterial pathogens but struggle with unculturable organisms.
  • Certain viral pathogens, such as hepatitis C virus (HCV), are not detectable by standard culture.

Purpose of the Study:

  • To highlight the evolution of diagnostic methods in evaluating disease pathogenesis and therapeutic responses.
  • To underscore the limitations of traditional culture-based pathogen detection.
  • To introduce the impact of molecular diagnostics on identifying infectious agents.

Main Methods:

  • Review of historical and current diagnostic approaches in clinical research and practice.
  • Comparison of culture-based methods versus molecular diagnostic techniques for pathogen detection.
  • Examples of viral pathogens with differing detectability via culture (e.g., HIV vs. HCV).

Main Results:

  • Molecular diagnostics offer direct measurement of infectious agents, including previously unculturable pathogens.
  • Advances in molecular techniques expand diagnostic capabilities beyond traditional culture limitations.
  • Improved detection methods facilitate more accurate disease assessment and treatment monitoring.

Conclusions:

  • Molecular diagnostics represent a significant advancement in infectious disease detection and management.
  • These methods enhance the ability to identify a broader range of pathogens, improving clinical decision-making.
  • The shift towards molecular diagnostics transforms the evaluation of disease pathogenesis and response to therapy.