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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Jun 4, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard.

O Lantz¹, E Bonney, F Griscelli

¹Inserm Unit, Necker Hospital, Paris, France.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Quantitative PCR (qPCR) offers two main methods: end-point and kinetic. This chapter details their characteristics, compares their pros and cons, and highlights kinetic qPCR applications.

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Area of Science:

Molecular Biology
Biochemistry

Background:

Quantitative PCR (qPCR) is a vital technique for measuring nucleic acid quantities.
Two primary approaches exist: end-point and kinetic quantitative PCR.

Purpose of the Study:

To define and differentiate end-point and kinetic quantitative PCR methods.
To compare the advantages and disadvantages of each quantitative PCR approach.
To present applications of the kinetic quantitative PCR method.

Main Methods:

End-point quantitative PCR: measures PCR product at a specific cycle.
Kinetic quantitative PCR: monitors product accumulation throughout the PCR cycles.

Main Results:

Detailed definitions and characteristics of both end-point and kinetic qPCR.
Comparative analysis of the strengths and weaknesses of each method.
Illustrative examples of kinetic qPCR in practical applications.

Conclusions:

Understanding the distinctions between end-point and kinetic qPCR is crucial for method selection.
Kinetic qPCR offers dynamic monitoring with potential advantages in certain applications.
The presented applications showcase the utility of kinetic qPCR in research.