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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 4, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

Basic immunocytochemistry for light microscopy.

S A Brooks1

  • 1Research School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Immunocytochemistry identifies cell antigens using specific antibodies and labels. Key developments include improved labels like horseradish peroxidase and sensitive detection methods.

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Last Updated: Jun 4, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Multiplex Cyclic Fluorescent Immunohistochemistry
04:21

Multiplex Cyclic Fluorescent Immunohistochemistry

Published on: January 26, 2024

Area of Science:

  • Immunocytochemistry
  • Cell Biology
  • Histology

Background:

  • Immunocytochemistry (ICC) identifies specific antigens within cells or tissues.
  • It relies on antibody-antigen interactions visualized with a microscopic label.
  • The technique requires antigen preservation, a suitable antibody, and an effective label.

Purpose of the Study:

  • To define immunocytochemistry and outline its fundamental requirements.
  • To trace the historical development of immunocytochemistry techniques.
  • To highlight significant advancements in immunocytochemistry.

Main Methods:

  • Original method: Direct antibody labeling with fluorescent tags (Coons et al.).
  • Enzyme-based labels: Use of horseradish peroxidase and alkaline phosphatase.
  • Advanced detection: Multilayer methods and avidin-biotin binding strategies.

Main Results:

  • Demonstration of antigen localization in tissue sections using immunofluorescence.
  • Development of enzyme-labeled antibodies for enhanced detection sensitivity.
  • Implementation of avidin-biotin systems for highly specific and sensitive signal amplification.

Conclusions:

  • Immunocytochemistry is a powerful tool for in situ antigen identification.
  • Continuous refinement has led to increased sensitivity and specificity.
  • Modern immunocytochemistry utilizes advanced labeling and detection strategies.