Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Published on: May 31, 2024

In vivo matrigel migration and angiogenesis assays.

¹Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD.

Methods in Molecular Medicine

|February 23, 2011

Summary

A new in vivo method is needed to quickly assess the potential of compounds to promote angiogenesis, the formation of new blood vessels. This technique will complement existing in vitro studies for various applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Thymosin beta4 accelerates wound healing.

The Journal of investigative dermatology·1999

Same author

Gp38k, a protein synthesized by vascular smooth muscle cells, stimulates directional migration of human umbilical vein endothelial cells.

Experimental cell research·1999

Same author

Identification of endothelial cell binding sites on the laminin gamma 1 chain.

Circulation research·1999

Same author

Identification of laminin alpha1 and beta1 chain peptides active for endothelial cell adhesion, tube formation, and aortic sprouting.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·1999

Same author

Cell binding sequences in mouse laminin alpha1 chain.

The Journal of biological chemistry·1998

Same author

Differential effects of hepatocyte growth factor isoforms on epithelial and endothelial tubulogenesis.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·1998

Area of Science:

Physiology
Biomedical Engineering
Pharmacology

Background:

Angiogenesis is crucial for development, healing, and diseases like cancer.
Existing methods for assessing angiogenic potential are often time-consuming.
A need exists for rapid in vivo validation of pro-angiogenic compounds.

Purpose of the Study:

To develop a simple and rapid in vivo assay for evaluating the angiogenic potential of compounds.
To provide a complementary method to in vitro assessments.
To facilitate the study of angiogenesis in various physiological and pathological contexts.

Main Methods:

The study describes the development and validation of a novel in vivo angiogenesis assay.
Details of the experimental setup and readout parameters are provided.

More Related Videos

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Rat Mesentery Angiogenesis Assay

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

Related Experiment Videos

Last Updated: Jun 4, 2026

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Published on: May 31, 2024

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Rat Mesentery Angiogenesis Assay

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

The method is designed for speed and ease of implementation.

Main Results:

The assay successfully quantifies the angiogenic response to known pro-angiogenic agents.
Results demonstrate the reliability and reproducibility of the in vivo method.
The assay's capacity to augment in vitro findings is highlighted.

Conclusions:

A new, efficient in vivo method for assessing compound angiogenic potential has been established.
This assay serves as a valuable tool for drug discovery and research in angiogenesis-related diseases.
The method offers a practical solution for researchers needing rapid in vivo validation.