Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Published on: November 30, 2009

Angiogenesis assays using chick chorioallantoic membrane.

D C West¹, W D Thompson, P G Sells

¹Department of Immunology,Faculty of Medicine, University of Liverpool, Liverpool, UK.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Maternal plasma cortisol's effect on offspring birth weight: a Mendelian Randomisation study.

BMC pregnancy and childbirth·2024

Same author

The Medico-Legal Aspects of Venereal Disease.

The British journal of venereal diseases·2011

Same author

Rat Aortic Ring : 3D Model of Angiogenesis In Vitro.

Methods in molecular medicine·2011

Same author

Location and conduction velocity of raphespinal neurones in nucleus raphe magnus and raphe pallidus in the cat.

Neuroscience letters·2009

Same author

Responses of raphespinal and other bulbar raphe neurones to stimulation of the periaqueductal gray in the cat.

Neuroscience letters·2009

Same author

Excising basal cell carcinomas: comparing the performance of general practitioners, hospital skin specialists and other hospital specialists.

Clinical and experimental dermatology·2008

Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015

Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014

Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014

Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014

Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014

Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014

See all related articles

Researchers explored various in vivo angiogenesis assays for studying blood vessel formation. The chick embryo chorioallantoic membrane (CAM) assay is highlighted as a simple, cost-effective, and widely used model for angiogenesis research.

Area of Science:

Biomedical research
Vascular biology
Drug discovery

Background:

Angiogenesis, the formation of new blood vessels, is crucial in development and disease.
Therapeutic strategies targeting angiogenesis are actively pursued.
Numerous in vivo models exist to study this complex process.

Purpose of the Study:

To review and compare various in vivo angiogenesis assays.
To identify the most suitable model for studying angiogenesis and anti-angiogenesis.

Main Methods:

Review of classical and recent in vivo angiogenesis assays.
Comparison of assays based on difficulty, quantification, speed, and cost.
Focus on the chick embryo chorioallantoic membrane (CAM) assay.

More Related Videos

Quail Chorioallantoic Membrane - A Tool for Photodynamic Diagnosis and Therapy

Quail Chorioallantoic Membrane - A Tool for Photodynamic Diagnosis and Therapy

Published on: April 28, 2022

The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer

The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer

Published on: June 21, 2019

Related Experiment Videos

Last Updated: Jun 4, 2026

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Published on: November 30, 2009

Quail Chorioallantoic Membrane - A Tool for Photodynamic Diagnosis and Therapy

Quail Chorioallantoic Membrane - A Tool for Photodynamic Diagnosis and Therapy

Published on: April 28, 2022

The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer

The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer

Published on: June 21, 2019

Main Results:

Classical models include rabbit ear chamber, hamster cheek pouch, and CAM assay.
Recent methods involve Matrigel/alginate plugs and sponges.
The CAM assay is the most widely utilized due to its simplicity and low cost.

Conclusions:

The CAM assay is a highly valuable and accessible model for angiogenesis research.
Its ease of use and affordability make it ideal for studying both pro-angiogenic and anti-angiogenic effects.