Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye
11:49

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

Corneal assay for angiogenesis.

M Ziche1

  • 1Institute of Pharmacological Sciences, University of Siena, Siena, Italy.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

The avascular cornea assay is a valuable animal model for studying new blood vessel growth (angiogenesis). This method quantifies in vivo angiogenesis by measuring new blood vessels in the initially avascular cornea.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence of β-blockers drug repurposing for the treatment of triple negative breast cancer: A systematic review.

Neoplasma·2019
Same author

ALDH2 Activity Reduces Mitochondrial Oxygen Reserve Capacity in Endothelial Cells and Induces Senescence Properties.

Oxidative medicine and cellular longevity·2018
Same author

Characterization of zofenoprilat as an inducer of functional angiogenesis through increased H2 S availability.

British journal of pharmacology·2015
Same author

EGFR signaling upregulates expression of microsomal prostaglandin E synthase-1 in cancer cells leading to enhanced tumorigenicity.

Oncogene·2011
Same author

A nanoscale fluorocarbon coating on PET surfaces improves the adhesion and growth of cultured coronary endothelial cells.

Nanotechnology·2011
Same author

Angiogenic phenotype induced by basic fibroblast growth factor transfection in brain microvascular endothelial cells.

International journal of oncology·2011

Area of Science:

  • Biomedical research
  • Vascular biology
  • Animal models

Background:

  • Developing effective angiogenic and antiangiogenic strategies requires precise animal models for in vivo angiogenesis analysis.
  • Various in vivo techniques exist, including chamber models, implants, and membrane assays.

Purpose of the Study:

  • To discuss the avascular cornea assay as a method for studying in vivo angiogenesis.
  • To evaluate the advantages and disadvantages of the cornea assay across different species.

Main Methods:

  • The cornea assay involves placing an angiogenic inducer into a corneal pocket.
  • This stimulates vascular outgrowth from the limbal vasculature.
  • The assay measures new blood vessel formation in the initially avascular cornea.

More Related Videos

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study
06:13

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study

Published on: August 18, 2011

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis
06:10

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Published on: August 6, 2020

Related Experiment Videos

Last Updated: Jun 4, 2026

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye
11:49

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study
06:13

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study

Published on: August 18, 2011

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis
06:10

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Published on: August 6, 2020

Main Results:

  • The cornea assay allows for the measurement of new blood vessels exclusively.
  • This is due to the cornea's naturally avascular state.
  • The assay is advantageous compared to other in vivo models for quantifying specific neovascularization.

Conclusions:

  • The avascular cornea assay is a quantitative and specific method for studying angiogenesis in vivo.
  • Its ability to measure only new blood vessels makes it a powerful tool for research.
  • Further understanding of its application across species can optimize its use in developing therapeutic strategies.