Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Overview of Cell-Matrix Interactions

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Persistently increased expression of the transforming growth factor-β1 gene in human vascular restenosis: Analysis of 62 patients with one or more episode of restenosis.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015

Same author

Use of human tissue specimens obtained by directional atherectomy to study restenosis.

Trends in cardiovascular medicine·2011

Same author

Laser Raman spectroscopy of atherosclerotic lesions in human coronary artery segments.

Applied optics·2010

Same author

Laser Raman spectroscopy of calcified atherosclerotic lesions in cardiovascular tissue.

Applied optics·2010

Same author

Therapeutic angiogenesis in critical limb and myocardial ischemia.

Journal of interventional cardiology·2002

Same author

[Cardiac and vascular gene therapy in cardiology. Current status and future prospects].

Der Internist·2002

Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015

Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014

Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014

Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014

Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014

Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Evaluation of angiogenesis.

M Silver¹, J M Isner

¹Departments of Medicine (Cardiology) and Biomedical Research, St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, MA.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Developing new therapies to stimulate blood vessel growth could prevent lower extremity amputations. This study introduces a mouse model to investigate the mechanisms of neovascularization in ischemic limbs.

More Related Videos

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Published on: May 31, 2024

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Published on: November 23, 2014

Related Experiment Videos

Last Updated: Jun 4, 2026

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Published on: May 31, 2024

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

Published on: November 23, 2014

Area of Science:

Vascular Biology
Regenerative Medicine
Animal Models

Background:

Severe lower extremity vascular disorders often necessitate amputation.
Stimulating blood vessel growth (neovascularization) offers a potential alternative to amputation.
Gene or protein therapies are emerging approaches for promoting neovascularization.

Purpose of the Study:

To investigate the mechanisms of lower-extremity collateral vessel development.
To establish a reliable murine model for studying angiogenesis in ischemic limbs.
To provide foundational data for future human clinical trials of neovascularization therapies.

Main Methods:

Developed a murine model by excising the femoral artery to induce limb ischemia.
Utilized laser Doppler perfusion imaging for in vivo monitoring of collateral blood vessel development.
Employed ex vivo immunohistochemistry to quantify capillary density in ischemic tissues.

Main Results:

Successfully created a murine model of limb ischemia.
Established methods for monitoring and quantifying neovascularization in response to ischemia.
Provided a platform for studying genetic factors influencing angiogenesis.

Conclusions:

The developed murine model is suitable for studying the basic mechanisms of lower-extremity angiogenesis.
This model can facilitate the investigation of key factors for neovascularization relevant to human vascular disorders.
Further research using this model can inform the development of novel therapeutic strategies to prevent amputation.