Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Histological validation of a type 1 diabetes clinical diagnostic model for classification of diabetes.

Diabetic medicine : a journal of the British Diabetic Association·2020

Same author

Factor X and its deficiency states.

Haemophilia : the official journal of the World Federation of Hemophilia·2016

Same author

Laboratory monitoring of haemostasis.

Anaesthesia·2014

Same author

Genetic testing in bleeding disorders.

Haemophilia : the official journal of the World Federation of Hemophilia·2014

Same author

Recurrent pregnancy loss and thrombophilia.

The Ceylon medical journal·2014

Same author

Pollen pool heterogeneity in jack pine (Pinus banksiana Lamb.): a problem for estimating outcrossing rates?

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Published on: August 21, 2016

Screening for Mutations in DNA by Single-Stranded Conformation Polymorphism (SSCP) Analysis.

¹Department of Haematology, Haemophilia Centre and Haemostasis Unit, Royal Free Hospital School of Medicine, London, UK.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Single-stranded DNA conformation analysis detects sequence variations. This method, single-stranded conformation polymorphism (SSCP) analysis, uses DNA fragment mobility shifts in gels to identify mutations.

More Related Videos

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Published on: August 21, 2016

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Single-stranded DNA (ssDNA) can adopt various conformations.
These conformations influence DNA fragment electrophoretic mobility.
Sequence-dependent secondary structures dictate ssDNA conformation.

Purpose of the Study:

To detect underlying sequence variations and mutations.
To explore the relationship between DNA sequence, conformation, and mobility.
To present modifications of single-stranded conformation polymorphism (SSCP) analysis.

Main Methods:

Electrophoretic separation of ssDNA fragments in nondenaturing gels.
Analysis of mobility shifts attributed to conformational changes.
Utilizing techniques like Mutation Detection Enhancement (MDE) gels for improved detection.

Main Results:

Sequence variations alter ssDNA conformation.
Differing conformations exhibit distinct electrophoretic mobilities.
Conformational differences allow for the detection of sequence variations.

Conclusions:

Single-stranded conformation polymorphism (SSCP) analysis is a valuable method for detecting DNA sequence variations.
Modifications to SSCP, such as using MDE gels, enhance mutation detection sensitivity.
The technique leverages sequence-dependent conformational changes in ssDNA.