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Related Concept Videos

RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Related Experiment Video

Updated: Jun 4, 2026

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
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Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells.

C Csortos1, V Lazar, J G Garcia

  • 1Department of Medicine, Indiana University School of Medicine, Indianapolis, IN.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Researchers screened a cDNA library to find a specific gene. This process identified a nonmuscle myosin light chain kinase in human cells, crucial for vascular permeability.

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Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
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Last Updated: Jun 4, 2026

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Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Screening complementary DNA (cDNA) libraries is essential for isolating specific genes.
  • Identifying novel gene isoforms aids in understanding complex cellular processes.
  • Myosin light chain kinase (MLCK) plays a critical role in smooth muscle contraction.

Purpose of the Study:

  • To isolate a specific cDNA clone encoding a protein of interest from a human umbilical vein endothelial cell cDNA library.
  • To identify the nonmuscle high molecular weight myosin light chain kinase (MLCK) isoform.
  • To elucidate the role of this specific MLCK isoform in endothelial cell function.

Main Methods:

  • cDNA library screening using established molecular biology techniques.
  • Selection of recombinant clones based on specific criteria.
  • Characterization of the isolated cDNA clone and its encoded protein.

Main Results:

  • Successful isolation of the nonmuscle high molecular weight MLCK isoform from the endothelial cell cDNA library.
  • Confirmation of the cDNA's ability to encode the target protein.
  • Demonstration of the MLCK isoform's role in phosphorylating myosin light chains.

Conclusions:

  • The study successfully identified a specific MLCK isoform, highlighting the power of cDNA library screening.
  • This nonmuscle MLCK isoform is vital for agonist-mediated endothelial cell contraction and vascular permeability.
  • The findings provide a foundation for further research into endothelial cell signaling pathways.