Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Global expression profiling of strains harbouring null mutations reveals that the five rpf-like genes of Mycobacterium tuberculosis show functional redundancy.

Tuberculosis (Edinburgh, Scotland)·2004

Same author

The Mycobacterium tuberculosis dosRS two-component system is induced by multiple stresses.

Tuberculosis (Edinburgh, Scotland)·2004

Same author

Effects of FTDP-17 mutations on the in vitro phosphorylation of tau by glycogen synthase kinase 3beta identified by mass spectrometry demonstrate certain mutations exert long-range conformational changes.

FEBS letters·2001

Same author

Use of two-dimensional gel electrophoresis to measure changes in synovial fluid proteins from patients with rheumatoid arthritis treated with antibody to CD4.

Clinical and diagnostic laboratory immunology·2001

Same author

Differential effects of apolipoprotein E isoforms on phosphorylation at specific sites on tau by glycogen synthase kinase-3 beta identified by nano-electrospray mass spectrometry.

FEBS letters·2000

Same author

Activation of the pro-drug ethionamide is regulated in mycobacteria.

The Journal of biological chemistry·2000

Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015

Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014

Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014

Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014

Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014

Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Proteomics.

J C Betts¹, M A Smith

¹Respiratory Systems, Glaxo Wellcome Research and Development, Hertfordshire, UK.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Proteomics technologies analyze the complete set of proteins (proteome), including expression and modifications. This aids in understanding Mycobacterium tuberculosis survival mechanisms.

Related Experiment Videos

Last Updated: Jun 4, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Biochemistry
Molecular Biology
Microbiology

Background:

Proteomics defines the complete protein set (proteome) of an organism.
It encompasses protein expression, posttranslational modifications, and cellular localization.
Technological advancements have spurred the growth of proteomics.

Purpose of the Study:

To describe the field of proteomics and its methodologies.
To highlight the application of proteomics in studying Mycobacterium tuberculosis.
To investigate mechanisms of M. tuberculosis survival within a host.

Main Methods:

Proteomics commonly integrates two-dimensional polyacrylamide gel electrophoresis (2D-PAGE).
Mass spectrometry and database searching are used for protein identification.
Genome sequencing data aids in proteomic analysis.

Main Results:

Recent advances in mass spectrometry enhance proteomic studies.
Availability of Mycobacterium tuberculosis genome sequences facilitates proteomic analysis.
Proteomic studies can elucidate bacterial survival strategies.

Conclusions:

Proteomics offers a comprehensive view of an organism's protein complement.
This approach is valuable for understanding microbial survival mechanisms.
Further proteomic research on M. tuberculosis can reveal host-pathogen interactions.