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DNA Microarrays

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Related Experiment Video

Updated: Jun 4, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method

Identification of Mycobacterium Species by DNA Microarray Chip Method

Published on: June 24, 2025

Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays.

M Wilson, M Voskuil, D Schnappinger

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Researchers developed a Mycobacterium tuberculosis microarray to analyze gene expression and compare bacterial strains. This tool enables genome-wide functional and comparative genomics for tuberculosis research.

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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

Area of Science:

Genomics
Microbiology
Molecular Biology

Background:

The completion of the Mycobacterium tuberculosis H37Rv genome sequence marked a milestone, enabling advanced genomic studies.
Microarray technology is a key tool in the post-genomic era for analyzing gene expression and genomic variations.

Purpose of the Study:

To describe the fabrication and application of a Mycobacterium tuberculosis microarray.
To demonstrate microarray-based transcript profiling for gene response analysis.
To showcase microarray-based comparative genomics for identifying genomic differences between strains.

Main Methods:

Fabrication of a microarray containing representations of all 3924 identified open reading frames (ORFs) of Mycobacterium tuberculosis.
Application 1: Transcript profiling to identify genes expressed under specific growth conditions or pathway inhibitions.
Application 2: Comparative genomics using the microarray to detect ORFs deleted or absent in other strains.

Main Results:

The study details the creation of a comprehensive M. tuberculosis microarray.
Two distinct applications of the microarray are presented: transcript profiling and comparative genomics.
These applications were successfully demonstrated using M. tuberculosis.

Conclusions:

The developed M. tuberculosis microarray is a powerful tool for genome-wide functional and comparative genomics.
Microarray technology facilitates the investigation of gene expression patterns and genomic diversity in Mycobacterium tuberculosis.
This approach aids in understanding the biological differences between M. tuberculosis strains.