Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells.

International journal of molecular sciences·2023

Same author

Cell cycle dependent expression of the CCK2 receptor by gastrointestinal myofibroblasts: putative role in determining cell migration.

Physiological reports·2017

Same author

Fluorescent measurement of [Ca²⁺]c: basic practical considerations.

Methods in molecular biology (Clifton, N.J.)·2012

Same author

Dynamic changes in cytosolic and mitochondrial ATP levels in pancreatic acinar cells.

Gastroenterology·2010

Same author

Compartmentalized signalling: Ca2+ compartments, microdomains and the many facets of Ca2+ signalling.

The FEBS journal·2009

Same author

Attenuation of receptor-dependent and -independent vasoconstriction in the human radial artery.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery·2008

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Published on: February 13, 2012

Fluorescent measurement of [Ca²+]c: basic practical considerations.

Alec W M Simpson¹

¹Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool, UK.

Methods in Molecular Biology (Clifton, N.J.)

|February 23, 2011

Summary

This summary is machine-generated.

Measuring cytosolic-free calcium (Ca2+) is complex due to diverse indicators, cell types, and equipment. This guide offers an overview to help researchers develop custom protocols for accurate Ca2+ measurements.

More Related Videos

Real-time Evaluation of Absolute, Cytosolic, Free Ca2+ and Corresponding Contractility in Isolated, Pressurized Lymph Vessels

Real-time Evaluation of Absolute, Cytosolic, Free Ca²⁺ and Corresponding Contractility in Isolated, Pressurized Lymph Vessels

Published on: March 22, 2024

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Published on: June 20, 2016

Related Experiment Videos

Last Updated: Jun 4, 2026

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Published on: February 13, 2012

Real-time Evaluation of Absolute, Cytosolic, Free Ca2+ and Corresponding Contractility in Isolated, Pressurized Lymph Vessels

Real-time Evaluation of Absolute, Cytosolic, Free Ca²⁺ and Corresponding Contractility in Isolated, Pressurized Lymph Vessels

Published on: March 22, 2024

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Published on: June 20, 2016

Area of Science:

Biochemistry
Cell Biology
Physiology

Background:

Accurate measurement of cytosolic-free calcium (Ca2+) is crucial for understanding cellular processes.
A wide array of fluorescent Ca2+ indicators, cell types, and detection equipment complicates standardized measurement protocols.

Purpose of the Study:

To provide an overview of the technologies available for measuring cytosolic-free Ca2+.
To guide researchers in developing experimental protocols tailored to their specific objectives, cell models, and available equipment.

Main Methods:

Discussion of various fluorescent Ca2+ indicators.
Consideration of different cell types for investigation.
Overview of available detection equipment for Ca2+ measurements.

Main Results:

Highlights the challenges in establishing a universal protocol for Ca2+ measurement.
Emphasizes the need for customized approaches based on experimental context.

Conclusions:

Researchers must develop specific protocols for measuring cytosolic-free Ca2+.
Protocol development should consider the choice of Ca2+ indicators, cell types, and detection systems.