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[Study of food products containing gluten by using the method of polymerase chain reaction]
Voprosy Pitaniia
|February 24, 2011
Abstract:
The results of studies on the detection of gluten in the 7 products, members of the group of gluten-free, used for patients with celiac disease. Used the method of polymerase chain reaction detection in real time (by method of polymerase chain reaction) using a test system SureFood Allergen Gluten. With this method, fully meets the requirements of the Codex Alimentarius Commission, confirmed the compliance of the investigated food gluten-free category, used for patients with celiac disease.
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PCR - Polymerase Chain Reaction
Overview
Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...

