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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 4, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Retracted: Platelet storage lesion: current proteomics approach.

Ashish Gupta¹, Tulika Chandra, Ashutosh Kumar

¹Department of Transfusion Medicine and Blood Bank, Chhatrapati Shahuji Maharaj Medical University, Lucknow, Uttar Pradesh, India.

|February 24, 2011

Summary

This summary is machine-generated.

Proteomics can identify changes in platelet proteins during storage, aiding in understanding and improving platelet quality. This research highlights protein kinases as key players in platelet storage lesion development.

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Last Updated: Jun 4, 2026

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Published on: November 29, 2024

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Published on: April 4, 2016

Area of Science:

Hematology
Transfusion Medicine
Proteomics

Background:

Platelet storage lesion (PSL) encompasses all detrimental changes to platelet structure and function during storage.
Proteomics offers a powerful approach for analyzing protein expression and function in biological systems.
Assessing platelet quality during storage is crucial, comparable in importance to managing bacterial contamination risks.

Purpose of the Study:

To explore the utility of proteomics in identifying and understanding the mechanisms of platelet storage lesion (PSL).
To investigate the impact of pathogen reduction technologies (PRT) on PSL development.
To identify potential therapeutic targets for mitigating PSL and extending platelet shelf-life.

Main Methods:

Quantitative proteomic techniques including differential gel electrophoresis, isotope-coded affinity tagging, and isotope tagging for relative and absolute quantitation.
Analysis of platelet proteomes using mass spectrometry.
Evaluation of the effects of UV-A/photosensitizer and UV-C treatments on platelet concentrates.

Main Results:

Proteomics has emerged as a valuable tool for identifying novel platelet proteins and analyzing proteome alterations during storage.
Pathogen reduction technologies (PRT), such as UV-A/photosensitizer and UV-C, can accelerate PSL development.
Protein kinases are implicated as significant contributors to PSL, suggesting them as potential targets for intervention.

Conclusions:

Proteomics provides a robust method for decoding complex biological processes, including PSL development.
Targeting protein kinases may offer a strategy to reduce PSL and improve platelet quality.
Extending platelet shelf-life by 2-3 days could significantly benefit inventory management, donor recruitment, and cost-efficiency.