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Updated: Jun 4, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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Published on: September 13, 2022

Advances and perspectives in cell-specific aptamers.

Wei Sun1, Lupei Du, Minyong Li

  • 1Department of Medicinal Chemistry, School of Pharmacy, Jinan, Shandong 250012, China.

Current Pharmaceutical Design
|February 24, 2011
PubMed
Summary

Nucleic acid aptamers, developed through Systematic Evolution of Ligands by Exponential enrichment (SELEX), are key in biomedicine. Cell-SELEX, an adaptation for living cells, offers promising analytical, therapeutic, and diagnostic applications.

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Last Updated: Jun 4, 2026

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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biomedical Engineering

Background:

  • Nucleic acid aptamers are versatile molecules selected via Systematic Evolution of Ligands by Exponential enrichment (SELEX).
  • Conventional SELEX has been widely applied in various biomedical fields.
  • Cell-specific aptamers derived from cell-SELEX are gaining traction for advanced applications.

Purpose of the Study:

  • To review contemporary advancements in cell-specific aptamer development.
  • To provide an overview of cell-SELEX methodology and its optimizations.
  • To highlight the diverse applications of cell-specific aptamers in biomedical research.

Main Methods:

  • Systematic Evolution of Ligands by Exponential enrichment (SELEX) for aptamer selection.
  • Cell-SELEX: an adaptation of SELEX for targeting whole living cells.
  • Review of current literature on cell-specific aptamer development and applications.

Main Results:

  • Cell-SELEX enables the generation of aptamers that recognize specific cell surface targets.
  • Optimized cell-SELEX protocols enhance aptamer affinity and specificity.
  • Cell-specific aptamers demonstrate significant potential in diagnostics, therapeutics, and analytical tools.

Conclusions:

  • Cell-SELEX is a powerful technology for developing targeted aptamers for specific cell populations.
  • Continued optimization of cell-SELEX methodologies will expand its utility.
  • Cell-specific aptamers are poised to revolutionize various biomedical applications, from diagnostics to targeted therapies.