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Published on: May 27, 2022
Application of three spectrometric methods to total selenium determination in postmortem material in a case of acute
Renata Wietecha-Posłuszny1, Teresa Lech, Paweł Kościelniak
1Laboratory for Forensic Chemistry, Faculty of Chemistry, Jagiellonian University, 3 Ingardena St., 30-060 Kraków, Poland. wietecha@chemia.uj.edu.pl
Abstract:
Three spectrometric methods, that is, spectrofluorimetry (SF), atomic absorption spectrometry with electrothermal atomization (ET-AAS), and atomic fluorescence spectrometry with hydride generation (HG-AFS) were used for the determination of total selenium in biological samples taken from postmortem material in a case of acute selenium compound poisoning. The precision of the SF, ET-AAS, and HG-AFS methods (RSD, n=10) was found to be in the ranges of 10.0-15.0, 3.0-6.0 and 1.0-1.5%, respectively, and the detection limit was 10.0, 4.0, and 0.1 μg/L of Se, respectively. In the case of HG-AFS, the analytical procedure takes less time and is less laborious than the other methods considered. The obtained results show the usefulness of the HG-AFS method as a supplementary analytical tool to the SF and ET-AAS methods with respect to the determination of selenium as well as the possibility of using this method as a primary one in forensic toxicology practice.
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