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[Immunobiological activities of Veillonella parvula isolated from infected root canals]
1Department of Endodontics, Kanagawa Dental College.
Abstract:
The primary etiological agent of all periapical lesions has long been considered to be bacterial. Furthermore, bacteria from infected root canals are potential antigens capable of initiating immunological reactions in periapical tissues. The purpose of this study, therefore, was clarify the immunological potentials of Veillonella parvula (V. parvula), which was frequently isolated from root canals with periapical lesion. Immunobiological activities of V. parvula sonic extracts were investigated on the enhancement of monocytes migration, induction of interleukin-1 (IL-1) production, mitogenicity and polyclonal activation of B cell. Following results were obtained: 1. Both LPS and protein of V. parvula sonic extracts strongly enhanced the activity of human peripheral monocytes migration. 2. Induction of IL-1 on C3H/HeN mice macrophage by V. parvula sonic extracts were stronger than that of S. typhimurium LPS as positive control. 3. It was found that mitogenicity of LPS from V. parvula on splenocytes was stronger than that of the protein, however mitogenicity on thymocytes was not shown in both preparation. 4. The polyclonal activation of B cell on splenocytes of BALB/c nu/nu mice by V. parvula was induced by the protein and LPS. 5. These findings indicate that both the protein and LPS from V. parvula have a regulation of immunobiological responses against macrophages and lymphocytes.
Insights
Veillonella parvula, a root canal bacterium, significantly boosts immune responses. Its components activate monocytes, macrophages, and B cells, influencing periapical lesion immunity.
Area of Science:
- Oral microbiology
- Immunology
Context:
- Periapical lesions are primarily caused by bacteria from infected root canals.
- These bacteria can act as antigens, triggering immune responses in surrounding tissues.
Purpose:
- To investigate the immunobiological potential of Veillonella parvula (V. parvula), a bacterium frequently found in root canals with periapical lesions.
- To assess V. parvula's effects on monocyte migration, interleukin-1 (IL-1) production, and lymphocyte activation.
Summary:
- V. parvula sonic extracts, including its lipopolysaccharide (LPS) and protein components, significantly enhanced human monocyte migration.
- V. parvula extracts induced higher levels of IL-1 production in mouse macrophages compared to Salmonella typhimurium LPS.
- V. parvula LPS demonstrated strong mitogenicity on splenocytes, while both LPS and protein induced polyclonal B cell activation in mice.
Impact:
- These findings highlight the immunoregulatory roles of V. parvula components (LPS and protein) in modulating immune responses involving macrophages and lymphocytes.
- Understanding these interactions is crucial for developing strategies to manage periapical inflammatory diseases.