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DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Nuclei micro-array FISH, a desirable alternative for MCL diagnosis.

Hongjuan Chu¹, Xiqun Han, Huiyong Jiang

¹Department of Pathology, Southern Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, People's Republic of China.

Annals of Hematology

|February 24, 2011

Summary

This summary is machine-generated.

Mantle cell lymphoma (MCL) diagnosis is challenging due to its similarity to other small B cell lymphomas. Detecting the t(11;14) translocation using nuclei micro-array FISH offers a highly sensitive and specific diagnostic method.

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Area of Science:

Hematology
Oncology
Molecular Diagnostics

Background:

Mantle cell lymphoma (MCL) is a rare B cell lymphoma subtype.
MCL diagnosis can be difficult due to overlapping features with other small B cell lymphomas (SBCLs).

Purpose of the Study:

To develop a sensitive and specific diagnostic method for MCL.
To differentiate MCL from other SBCLs, including SLL, FL, MZL, and MALT.

Main Methods:

Morphologic and immunophenotypic analysis.
Polymerase chain reaction (PCR) for t(11;14) translocation.
Interphase nuclei micro-array fluorescence in situ hybridization (FISH) for t(11;14) translocation.

Main Results:

Cyclin D1 positivity was significantly higher in MCL (76.47%) compared to other SBCLs.
PCR detected t(11;14) translocation in 25.81%-35.48% of MCL cases.
Nuclei micro-array FISH detected t(11;14) translocation in 93.10% of MCL cases with strong signals.

Conclusions:

The t(11;14) translocation is a valuable diagnostic marker for MCL.
Nuclei micro-array FISH is a sensitive, specific, convenient, and economical method for detecting t(11;14) in MCL diagnosis.