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Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
Published on: January 14, 2021
Cellular integration and vascularisation promoted by a resorbable, particulate-leached, cross-linked
Simon C Baker1, Geraldine Rohman, Jennifer Hinley
1Jack Birch Unit of Molecular Carcinogenesis, Department of Biology, University of York, York, UK.
None:
Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200 µm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68(+) macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.
