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Mouse Pneumonectomy Model of Compensatory Lung Growth

Mouse Pneumonectomy Model of Compensatory Lung Growth

Published on: December 17, 2014

A modified technique for partial pneumonectomy in the mouse.

Sha-Ron Jackson¹, Genevieve N Williams, Jooeun Lee

¹Department of Pediatric Surgery, Developmental Biology Program and Regenerative Medicine, The Saban Institute for Research, Children's Hospital Los Angeles, University of Southern California School of Medicine, Los Angeles, California 90027, USA.

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research

|February 25, 2011

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A modified partial pneumonectomy (PNX) technique in mice significantly improves animal survival. This streamlined surgical method, using a neuro clip for lung resection, enhances recovery and reduces complications in lung repair research.

Area of Science:

Surgical techniques
Animal models
Pulmonary research

Background:

Partial pneumonectomy (PNX) in mice is a valuable model for studying lung repair and compensatory lung growth.
Existing PNX techniques face challenges that can impact animal survival.
A modified surgical approach is presented to improve outcomes.

Purpose of the Study:

To provide a detailed description of a modified murine partial pneumonectomy (PNX) technique.
To enhance animal survival rates during and after the PNX procedure.
To optimize the PNX model for lung repair research.

Main Methods:

Mice underwent anesthesia and mechanical ventilation via tracheotomy.
A left lateral thoracotomy allowed access to the left lung.

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Last Updated: Jun 4, 2026

Mouse Pneumonectomy Model of Compensatory Lung Growth

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Published on: December 17, 2014

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

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The lung was resected using a 5-mm neuro clip, with the procedure optimized for completion within 20 minutes.

Main Results:

The modified PNX technique achieved an intraoperative survival rate exceeding 85%.
Postoperative survival rates reached over 90% within seven days in wild-type C57BL/6J mice.
The use of a neuro clip streamlined the procedure compared to traditional silk suture methods.

Conclusions:

Minimizing surgical time is crucial for improving survival in murine PNX.
Employing a 5-mm neuro clip for lobe isolation simplifies the procedure and reduces cardiac arrhythmias.
This modified technique leads to significantly higher intraoperative and immediate postoperative survival rates, enhancing its utility in lung repair studies.