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A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Published on: June 26, 2020
Molecular virology of hepatitis E virus
Imran Ahmad1, R Prasida Holla, Shahid Jameel
1Virology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, India.
Virus Research
|February 25, 2011
Summary
This review covers the molecular virology of the hepatitis E virus (HEV). New in vitro systems verify existing knowledge and refine the HEV life cycle model.
Area of Science:
- Molecular Virology
- Hepatitis E Virus Research
- RNA Virus Biology
Background:
- Hepatitis E virus (HEV) research has advanced significantly.
- Information was previously derived from comparative studies and subgenomic expression.
- Recent development of replicons and in vitro infection systems allows for direct verification.
Purpose of the Study:
- To review the current understanding of HEV molecular virology.
- To detail the HEV genome and its open reading frames (ORFs): ORF1, ORF2, and ORF3.
- To propose a modified model of the HEV life cycle based on new data.
Main Methods:
- Review of existing literature on HEV molecular virology.
- Analysis of data from HEV replicons and in vitro infection systems.
- Comparative analysis with other positive-strand RNA viruses.
Main Results:
- Recent in vitro systems are confirming previously inferred data on HEV.
- Detailed characterization of HEV genome and proteins (ORF1, ORF2, ORF3) is presented.
- An updated model for the HEV life cycle is proposed.
Conclusions:
- The availability of replicons and in vitro systems has greatly enhanced HEV research.
- Current knowledge on HEV genome and proteins is consolidated.
- The revised HEV life cycle model provides a better framework for future studies.
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