Related Experiment Video
Updated: Aug 10, 2026
Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection
Published on: March 14, 2019
[Studies on suppurative lesion and bone distribution of peroral antimicrobial agents (LAPC and TFLX) to experimental
1Department of Oral and Maxillofacial Surgery I, Nippon Dental University School of Dentistry at Tokyo.
Abstract:
The models of infection in the rabbits mandible using Streptococcus milleri and Bacteroides fragilis by the method of Satoh-Heimdahl were prepared. A penicillic peroral antimicrobial agent, lenamipicillin (LAPC), and pyridone carboxylic acid peroral antimicrobial agent, tosufloxacin tosilate (TFLX), were administered to infected and healthy groups. Drug concentrations in the serum, pus, mandible, maxilla, humerus, sternum, costa, ilium, femur, tibia, liver, and kidney were determined by a biological method. The data were submitted to pharmacodynamic analysis and compared. The following results were obtained. The concentration of LAPC in the serum and various bone tissues was 1.2-6.4 times higher in the infected group than in the healthy group. The concentration of TFLX in the serum and bone tissues was 1.1-3.7 times higher in the infected group than in the healthy group. Moreover, concentrations in the liver 1.1-fold and 2.1-fold higher in the infectious group than in the healthy group in the LAPC and TFLX administration groups, respectively, and 1.1-fold and 1.3-fold higher in the kidney in the LAPC and TFLX groups, respectively. Both LAPC and TFLX diffused to the pus more satisfactorily than to the serum. The Cmax ratios of pus to infected serum were 1.48 and 1.81 for LAPC and TFLX, respectively, and the AUC ratios of pus to infected serum were 1.31 and 1.62 in LAPC and TFLX, respectively. These results indicated that both LAPC and TFLX are distributed satisfactorily to the local foci in the mandible, and that their systemic diffusion exerted immediate clinical effects.
Insights
Lenamipicillin (LAPC) and tosufloxacin tosilate (TFLX) showed higher concentrations in infected rabbit mandibles and pus compared to healthy subjects. Both antibiotics effectively diffused to infection sites, indicating promising clinical efficacy for treating mandibular infections.
Area of Science:
- Pharmacology
- Infectious Diseases
- Oral Surgery
Context:
- Mandibular infections pose significant clinical challenges.
- Accurate drug delivery to infection foci is crucial for treatment success.
- Animal models are essential for preclinical evaluation of antimicrobial agents.
Purpose:
- To evaluate the pharmacokinetics and tissue distribution of lenamipicillin (LAPC) and tosufloxacin tosilate (TFLX) in a rabbit mandibular infection model.
- To compare drug concentrations in serum, pus, bone, and organs between infected and healthy rabbits.
- To assess the diffusion of LAPC and TFLX into purulent exudate.
Summary:
- Infection models in rabbit mandibles were established using *Streptococcus milleri* and *Bacteroides fragilis*.
- LAPC and TFLX concentrations were significantly higher in serum and bone tissues of infected rabbits compared to healthy controls.
- Both antibiotics demonstrated superior diffusion into pus compared to serum, with favorable Cmax and AUC ratios.
Impact:
- The findings suggest that both LAPC and TFLX achieve satisfactory concentrations at local infection sites in the mandible.
- Systemic diffusion and local accumulation of these antibiotics indicate potential for effective clinical treatment of mandibular osteomyelitis.
- This study provides valuable pharmacokinetic data supporting the use of LAPC and TFLX in treating similar oral and maxillofacial infections.
