Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy.

Microscopy (Oxford, England)·2026
Same author

Microtubule Deformation Modulates Intracellular Transport by Kinesin Differently Than Dynein.

IEEE transactions on nanobioscience·2025
Same author

Short Repeat Ribonucleic Acid Reduces Cytotoxicity by Preventing the Aggregation of TDP-43 and Its 25 KDa Carboxy-Terminal Fragment.

JACS Au·2024
Same author

Hetero-oligomerization of TDP-43 carboxy-terminal fragments with cellular proteins contributes to proteotoxicity.

Communications biology·2024
Same author

Axonal transport of Frizzled5 by Alcadein α-containing vesicles is associated with kinesin-1.

Molecular biology of the cell·2023
Same author

Increased intracellular crowding during hyperosmotic stress.

Scientific reports·2023

Related Experiment Video

Updated: Jun 4, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

[Study of protein function using fluorescence correlation spectroscopy]

Masataka Kinjo1

  • 1Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University, 001-0021 Sapporo, Japan.

Seikagaku. the Journal of Japanese Biochemical Society
|February 26, 2011
PubMed
Summary

No abstract available in PubMed .

More Related Videos

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018