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Updated: Jun 4, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
Published on: June 25, 2020
Mass spectrometry characterization of plant phosphoproteins
Kateŕina Nováková1, Ondrej Sedo, Zbyněk Zdráhal
1Department of Functional Genomics and Proteomics, Faculty of Science, CEITEC, Masaryk University, Brno, Czech Republic.
Abstract:
Protein phosphorylation constitutes a major type of post-translational modifications mobilizing a high number of genes. It is involved in many crucial cell processes and largely participates in the features of the proteome. For several biological and technical reasons, the characterization of protein phosphorylation requires a combination of distinct procedures. In this review, a special emphasis is given to analytical strategies connected with the determination of the presence of phosphorylation sites and their localization by mass spectrometry (MS). The feasibility of selected combinations of analytical approaches for diverse objectives of phosphoproteomic research is discussed.
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