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Antioxidant effect of pomegranate extract in reducing acute inflammation due to myringotomy
1Otorhinolaryngology Department, Bezmialem Vakif University Medical Faculty, Istanbul, Turkey.
Objective:
To assess the effect of pomegranate extract on acute inflammation due to myringotomy.
Design:
Prospective, randomised study.
Subjects:
Thirty Sprague-Dawley rats were divided into three groups. Group one constituted controls. Group two underwent myringotomy. Group three underwent myringotomy and also received 100 µl/day pomegranate extract, via gavage, one day before and two days after surgery. Following sacrifice 48 hours after myringotomy, the animals' right ears were used to determine the concentration of reactive oxygen species, using the chemiluminescence method; left ears were used for histological study.
Results:
Reactive oxygen species levels were significantly decreased in group three compared with group two (p < 0.01). The density of inflammatory cells in group three was significantly less than that in group two (p < 0.01). Lamina propria thickness and vessel density were also significantly decreased in group three compared with group two (p < 0.01).
Conclusion:
Our results indicate that oral pomegranate extract decreases reactive oxygen species concentration and acute inflammation in the tympanic membrane after myringotomy.
Insights
Oral pomegranate extract significantly reduced inflammation and reactive oxygen species (ROS) in rats following myringotomy surgery. This natural compound shows promise for managing acute tympanic membrane inflammation.
Area of Science:
- Otolaryngology
- Inflammation Research
- Natural Product Therapeutics
Background:
- Myringotomy, a surgical procedure involving an incision in the tympanic membrane, can lead to acute inflammation.
- Reactive oxygen species (ROS) play a significant role in inflammatory processes.
- Pomegranate extract is recognized for its antioxidant and anti-inflammatory properties.
Purpose of the Study:
- To investigate the efficacy of oral pomegranate extract in mitigating acute inflammation and reducing ROS levels in the tympanic membrane after myringotomy.
- To evaluate the anti-inflammatory effects of pomegranate extract in a rodent model.
Main Methods:
- A prospective, randomized study was conducted on thirty Sprague-Dawley rats.
- Rats were divided into three groups: control, myringotomy only, and myringotomy with daily oral administration of pomegranate extract.
- Reactive oxygen species concentration and histological changes (inflammatory cell density, lamina propria thickness, vessel density) were assessed 48 hours post-myringotomy.
Main Results:
- Pomegranate extract administration significantly decreased reactive oxygen species levels compared to the myringotomy-only group (p < 0.01).
- Histological analysis revealed a significant reduction in inflammatory cell density, lamina propria thickness, and vessel density in the pomegranate extract group (p < 0.01).
Conclusions:
- Oral administration of pomegranate extract effectively reduces reactive oxygen species concentration in the tympanic membrane following myringotomy.
- Pomegranate extract demonstrates significant anti-inflammatory effects, decreasing key markers of acute inflammation in the tympanic membrane post-myringotomy.
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