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Updated: Jun 4, 2026
Isolation and Culture of Primary Human Mammary Epithelial Cells
Published on: May 3, 2024
Yeasts isolation from bovine mammary glands under different mastitis status in the Mexican High Plateu
Carolina Segundo Zaragoza1, Roberto Arnulfo Cervantes Olivares, Andrés Ernesto Ducoing Watty
1Departamento de Microbiología e Inmunología, Laboratorio de Micología Veterinaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Mexico. c segund@yahoo.com.mx
Background:
The mastitis subclinical and clinical in cows caused by fungi has been increased specially by yeast of the genus Candida.
Objective:
To identify what yeasts were present in milk samples obtained from mammary glands of healthy cows, and others suffering subclinical or clinical mastitis.
Methods:
From a total of 1,095 milk samples 342 were from mammary glands of healthy dairy cows, 383 with subclinical mastitis, and 370 with clinical mastitis, were taken, in the states of Querétaro, Hidalgo, Puebla and Mexico City (Distrito Federal) in the so called Mexican High Plateu. The clinical status of the mammary glands was determined by clinical examination and the California Mastitis Test. Yeasts identification was carried out by morphology and biochemical methods.
Results:
Twenty different species of Candida were identified out of 282 (25.75%) milk samples. The most frequently identified species in the healthy cows and cows with clinical mastitis groups were Candida glabrata and Candida krusei. On the other hand, samples from the subclinical mastitis group showed a diversity of Candida species, including Candida zeylanoides, Candida norvegica, Candida viswanathii, Candida guilliermondii, and Candida tropicalis. Candida albicans was isolated only in 11 (3.9%) samples from the clinical and subclinical mastitis groups.
Conclusions:
These results suggest the possible role that Candida species other than C. albicans may play in mycotic mastitis in cows.
Insights
Fungal mastitis in cows is increasing, primarily due to Candida yeasts. This study identified various Candida species in milk from healthy and mastitic cows, suggesting non-albicans species may contribute to mycotic mastitis.
Area of Science:
- Veterinary Mycology
- Dairy Cattle Health
- Infectious Diseases
Background:
- Fungal mastitis, particularly yeast infections caused by Candida species, is a growing concern in dairy cows.
- Understanding the prevalence and diversity of Candida species is crucial for effective management.
Purpose of the Study:
- To identify yeast species in milk from healthy, subclinically mastitic, and clinically mastitic cows.
- To investigate the role of different Candida species in bovine mastitis.
Main Methods:
- Analysis of 1,095 milk samples from dairy cows across four Mexican states.
- Determination of mammary gland health using clinical examination and the California Mastitis Test.
- Identification of yeast species through morphological and biochemical characterization.
Main Results:
- Twenty different Candida species were identified in 25.75% of milk samples.
- Candida glabrata and Candida krusei were most common in healthy and clinical mastitis cases.
- Subclinical mastitis cases showed a wider range of species, including Candida zeylanoides and Candida tropicalis.
- Candida albicans was found in only 3.9% of mastitic samples.
Conclusions:
- The study highlights the significant presence of diverse Candida species in bovine mastitis.
- Non-albicans Candida species may play a more substantial role in mycotic mastitis than previously recognized.
- Further research into the pathogenicity of these species is warranted.
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