Related Experiment Video
Updated: Jun 4, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Towards a knowledge-based correction of iron chlorosis
Javier Abadía1, Saúl Vázquez, Rubén Rellán-Álvarez
1Department of Plant Nutrition, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), P.O. BOX 13034, E-50080 Zaragoza, Spain. jabadia@eead.csic.es
Iron deficiency causes crop chlorosis and yield loss, especially in fruit trees on calcareous soils. This review covers iron fertilization strategies and plant iron uptake, transport, and utilization to optimize crop nutrition and reduce environmental impact.
Area of Science:
- Plant nutrition and soil science
- Agricultural chemistry
- Crop physiology
Background:
- Iron deficiency is a major nutritional disorder in crops, particularly fruit trees on calcareous soils, leading to chlorosis, reduced growth, and yield losses.
- Current agricultural practices rely on iron (Fe) fertilizers to manage Fe deficiency, with both soil and foliar application methods employed annually.
- While basic knowledge of plant Fe uptake, transport, and allocation exists, research on Fe-fertilized plants (resupplied with Fe) remains limited.
Purpose of the Study:
- To review recent advancements in iron fertilizer research.
- To synthesize the current understanding of iron acquisition, transport, and utilization in plants.
- To examine the impact of iron fertilization on plant responses to iron deficiency.
Main Methods:
- Literature review of recent developments in Fe-fertilizer research.
- Synthesis of existing knowledge on plant Fe homeostasis, acquisition, transport, and utilization.
- Analysis of studies on plant responses to Fe deficiency and Fe fertilization.
Main Results:
- Significant knowledge exists on plant Fe homeostasis, but studies on Fe-fertilized plants are scarce.
- Iron fertilization practices can be improved by integrating fundamental knowledge of plant Fe metabolism.
- Understanding plant Fe dynamics is crucial for optimizing fertilizer use and minimizing environmental impact.
Conclusions:
- Agronomic Fe-fertilization practices can be enhanced by leveraging basic research on plant Fe homeostasis.
- Optimizing fertilizer inputs through better understanding of plant Fe dynamics can reduce grower costs.
- Minimizing the environmental impact of fertilization is a key long-term goal achievable through improved agronomic practices.
Related Concept Videos
Microbes and Other Elemental Cycles
Key Elements for Plant Nutrition
Corrosion
Microbial Leaching
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...

