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Updated: Jun 4, 2026

Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
Published on: February 4, 2017
An alternative method for determining the radiochemical purity of 99mTc-tetrofosmin
Kanak C Amin1, Sagar Patel, Aniruddha Doke
1Department of Nuclear Medicine (Jb-3), Cleveland Clinic Foundation, Cleveland, Ohio, USA.
Abstract:
The instant thin-layer chromatographic method of determining the radiochemical purity of (99m)Tc-tetrofosmin recommended by the manufacturer uses a mixture of acetone and dichloromethane (35:65 v/v) as the solvent and a silica gel (SG) strip as the solid phase. Currently, SG strips are not available commercially and an alternative solid phase is needed. The present study reports that silicic acid strips are an appropriate substitute for SG strips for the chromatographic method using the same solvent mixture but mixed in a reverse ratio (65:35 v/v). The 2 methods yielded similar values (within 1 SD) of radiochemical purity for (99m)Tc-tetrofosmin.
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