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Updated: Jun 4, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Heme iron state in various oxyhemoglobins probed using Mössbauer spectroscopy with a high velocity resolution
M I Oshtrakh1, A L Berkovsky, A Kumar
1Faculty of Physical Techniques and Devices for Quality Control, Ural Federal University, Ekaterinburg, Russian Federation. oshtrakh@mail.utnet.ru
Abstract:
A comparative study of oxyhemoglobins from pig, rabbit, normal human and patients with blood system malignant diseases was performed using Mössbauer spectroscopy with a high velocity resolution at 90 K. Mössbauer spectra were fitted with the help of two models: using one quadrupole doublet (model of equivalent iron electronic structure in α- and β-subunits of hemoglobins) and superposition of two quadrupole doublets (model of non-equivalent iron electronic structure in α- and β-subunits of hemoglobins). The results obtained using both models demonstrated small variations of hyperfine parameters that were related to the heme iron state variation in different hemoglobins. These results were compared with structural and functional differences of the hemoglobins investigated.
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