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Updated: Jun 4, 2026

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
Published on: March 7, 2025
Use of NMR to Study H. pylori Metabolism
P A Chalk1, A D Roberts, A A Davison
1Exploratory Chemistry Unit, Glaxo Wellcome Research and Development, Stevenage, UK.
Abstract:
Until recently, the culture of Helicobacter pylori in vitro has only been achievable with complex undefined media supplemented with blood products, such as Brain-heart infusion (BHI) medium, blood agar, chocolate agar, or lysed blood agar (1). Substrate utilization and metabolic activity during growth on these media is difficult to define; this has led to the use of techniques such as nuclear magnetic resonance (NMR) spectroscopy to investigate the metabolic pathways active in H. pylori (e.g., refs. 2-5). Defined media for the growth of H. pylori have now been described (6,7), however, and may facilitate future metabolic studies in this organism by more conventional techniques.
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