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[Study of the expression level of zinc finger factor osterix associated with fluoride bone injury]
Junxiang Ma1, Yue Song, Kejian Liu
1Dpartment of Occupational and Environmental Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Objective:
To observe the expression of zinc finger transcription factor Osterix (Osx) in different groups of floride (F) exposure in order to investigate its role in the cause of fluoride bone injuries and ossification around bone.
Methods:
Western blot and fluorescence real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expressions of Osx mRNA and its protein in fibroblast, and the concentrations of F was 0.01, 0.1, 10 mg/L in experimental groups, and the control was 0 mg/L.
Results:
The levels of Osx protein in F exposure groups increased at first and then decreased with the levels of F exposure, in which a significant increases were found after fibroblasts were exposed to F for about 24-48 h in all experimental groups (P < 0.05). In comparition with the 0 h F exposure group, the trend of Osx was the same, the results of RT-PCR indicated that the levels of Osx mRNA significantly increased in the group of 24-48 h F exposure groups (P < 0.05), overall, the trends of Osx mRNA level were similar to the trend of Osx protein, which went up at first and then went down in each group.
Conclusion:
The expressions of Osx factor in fibroblast could be promoted by fluoride, which could play an important role in the process of the transformation from fibroblasts to osteoblast induced by fluoride, and in the development of fluoride bone injuries and ossification around bone.
