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Updated: Jun 4, 2026
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
Nonresonant femtosecond laser vaporization with electrospray postionization for ex vivo plant tissue typing using
Elizabeth J Judge1, John J Brady, Paolo Emilio Barbano
1Temple University, Department of Chemistry, 1901 North 13th Street, Philadelphia, Pennsylvania 19122, United States.
Abstract:
Laser electrospray mass spectrometry (LEMS) with offline classification is used to discriminate plant tissues at atmospheric pressure using an intense (10(13) W cm(-2)), nonresonant (800 nm) femtosecond laser pulse to vaporize cellular content for subsequent mass analysis. The tissue content of the plant within the 0.05 mm(2) laser interaction region is vaporized into the electrospray plume where the molecules are ionized prior to transfer into the mass spectrometer. The measurements for a flower petal, leaf, and stem of an impatiens plant reveal mass spectral signatures that enable discrimination as performed using a compressive linear classifier. The statistical analysis of the plant tissue samples reveals reproducibility of the data for replicate tissue samples and within a single tissue sample. A similar degree of discrimination was achieved for the green and white regions of aphelandra squarrosa (zebra plant) leaves.
