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Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue
Published on: April 6, 2022
A novel tissue-engineered approach to problems of the postpneumonectomy space
Nobuo Tsunooka1, Shin Hirayama, Jeffrey A Medin
1Latner Thoracic Surgery Research Laboratories, Division of Thoracic Surgery, Toronto General Hospital, University Health Network, Toronto, Ontario, Canada.
The Annals of Thoracic Surgery
|March 1, 2011
Summary
Engineered collagen matrices with cells show potential for treating post-pneumonectomy space complications. These cellularized matrices promote neovascularization and aid bacterial clearance, offering a less disfiguring alternative.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Thoracic Surgery
Background:
- Pleural space complications after pneumonectomy are challenging.
- Current treatments like tissue flaps and thoracoplasty are disfiguring.
- Novel tissue engineering approaches are needed.
Purpose of the Study:
- To investigate the feasibility of engineered tissue for post-pneumonectomy space complications.
- To evaluate cell survival, angiogenesis, and bacterial clearance in engineered matrices.
- To explore a less disfiguring treatment option.
Main Methods:
- Left pneumonectomy in mice followed by immediate implantation of cellularized collagen matrices.
- Bioluminescence imaging to assess cell viability and angiogenesis.
- Histology, immunohistochemistry, and flow cytometry for cellular analysis.
- Empyema model with Staphylococcus aureus to evaluate bacterial clearance.
Main Results:
- Cells survived and proliferated within collagen matrices in vivo.
- Significant neovascularization and increased total cellularity observed.
- Both cellularized and acellular matrices demonstrated bacterial clearance.
- Early cell survival and matrix integration confirmed.
Conclusions:
- Engineered cellularized collagen matrices are feasible for post-pneumonectomy space.
- These matrices support cell survival, neovascularization, and bacterial clearance.
- This approach offers a promising, less disfiguring alternative for managing post-pneumonectomy space issues.
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