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Related Concept Videos

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Updated: Jun 4, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Published on: November 4, 2016

Microarray-based genotyping of Staphylococcus aureus isolates from camels.

Stefan Monecke1, Ralf Ehricht, Peter Slickers

  • 1Institute for Medical Microbiology and Hygiene, Faculty of Medicine Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. monecke@rocketmail.com

Veterinary Microbiology
|March 1, 2011
PubMed
Summary
This summary is machine-generated.

This study genotyped Staphylococcus aureus from camels in Dubai, revealing common clonal complexes and virulence genes like leukocidin. Resistance markers were rare in these camel isolates.

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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
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Published on: September 5, 2013

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Camel Infectious Diseases

Background:

  • Staphylococcus aureus is a significant pathogen causing mastitis and other diseases in dromedary camels.
  • Understanding the genetic diversity and virulence factors of S. aureus in camels is crucial for disease management.

Purpose of the Study:

  • To investigate the population structure of Staphylococcus aureus isolates from dromedary camels in Dubai, UAE.
  • To characterize the carriage of toxin genes and antibiotic resistance markers within these S. aureus strains.

Main Methods:

  • Genotyping of 45 S. aureus isolates from dromedary camels using multilocus sequence typing (MLST).
  • Detection of specific toxin genes (e.g., leukocidin, staphylokinase, enterotoxins) and antibiotic resistance genes (e.g., beta-lactamase, tetK, mecA).

Main Results:

  • Dominant clonal complexes identified were CC6 (44.44%) and CC30 (35.56%), with a novel sequence type (ST1755) also described.
  • Resistance genes were infrequent; only 6.67% carried the beta-lactamase operon and 6.67% carried the tetK gene. The mecA gene was absent.
  • Commonly detected virulence markers included leukocidin genes (lukD+lukE, 55.56%), staphylokinase (sak, 48.89%), the enterotoxin gene cluster (egc, 33.33%), and a variant of enterotoxin A (sea-320E, 28.89%).

Conclusions:

  • This study provides the first comprehensive genotyping data on S. aureus population structure in Middle Eastern camels.
  • The findings highlight the prevalence of specific clonal complexes and virulence factors, with a low prevalence of antibiotic resistance markers in this camel population.