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p53 is important for the anti-invasion of ganoderic acid T in human carcinoma cells

Nian-Hong Chen1, Jian-Jiang Zhong

  • 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
|March 1, 2011
PubMed

Insights

Ganoderic acid T (GA-T) inhibits colon tumor cell invasion by promoting aggregation and suppressing migration. The tumor suppressor p53 protein is crucial for GA-T

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Pharmacology

Background:

  • Previous studies showed Ganoderic acid T (GA-T) inhibits tumor invasion and metastasis.
  • The specific role of p53 in GA-T's anti-invasion effects remained unclear.

Purpose of the Study:

  • To investigate the function of p53 in GA-T-induced anti-invasion in human colon tumor cells.
  • To elucidate the molecular mechanisms underlying GA-T's anti-invasion activity mediated by p53.

Main Methods:

  • Utilized HCT-116 p53(+/+) and p53(-/-) human colon tumor cell lines.
  • Assessed cell aggregation, adhesion, and migration.
  • Analyzed NF-κB pathway components, including nuclear translocation and IκBα degradation.
  • Quantified protein expression of uPA, MMP-2/9, iNOS/NOS2, and nitric oxide (NO) production.

Main Results:

  • GA-T dose-dependently promoted cell aggregation and inhibited cell migration and adhesion in both p53(+/+) and p53(-/-) cells.
  • p53 significantly modified GA-T's effects on cell aggregation, migration, and adhesion.
  • p53 modulated GA-T's inhibition of NF-κB signaling, uPA, MMP-2/9, iNOS/NOS2 expression, and NO production.

Conclusions:

  • p53 plays a critical role in GA-T's anti-invasion effects in human carcinoma cells.
  • p53 is a key molecular target for GA-T in inhibiting human carcinoma cell invasion.

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