Related Experiment Video
Updated: Jun 4, 2026

Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization
Published on: October 24, 2025
Associations between free fatty acids, cumulus oocyte complex morphology and ovarian function during in vitro
Emily S Jungheim1, George A Macones, Randall R Odem
1Department of Obstetrics and Gynecology, Washington University, St. Louis, Missouri 63108, USA. jungheime@wudosis.wustl.edu
Objective:
To determine if follicular free fatty acid (FFA) levels are associated with cumulus oocyte complex (COC) morphology.
Design:
Prospective cohort study.
Setting:
University in vitro fertilization (IVF) practice.
Patient(S):
A total of 102 women undergoing IVF.
Intervention(S):
Measurement of FFAs in serum and ovarian follicular fluid.
Main Outcome Measure(S):
Total and specific follicular and serum FFA levels, correlations between follicular and serum FFAs, and associations between follicular FFA levels and markers of oocyte quality, including COC morphology.
Result(S):
Predominant follicular fluid and serum FFAs were oleic, palmitic, linoleic, and stearic acids. Correlations between follicular and serum FFA concentrations were weak (r=0.252, 0.288, 0.236, 0.309, respectively for specific FFAs; r=0.212 for total FFAs). A receiver operating characteristic curve determined total follicular FFAs≥0.232 micromol/mL distinguished women with a lower versus higher percentage of COCs with favorable morphology. Women with elevated follicular FFAs (n=31) were more likely to have COCs with poor morphology than others (n=71; OR 3.3, 95% CI1.2-9.2). This relationship held after adjusting for potential confounders, including age, body mass index, endometriosis, and amount of gonadotropin administered (β=1.2; OR 3.4, 95% CI 1.1-10.4).
Conclusion(S):
Elevated follicular FFA levels are associated with poor COC morphology. Further work is needed to determine what factors influence follicular FFA levels and if these factors impact fertility.
More Related Videos
08:06The Immediate Partial Removal of Cumulus-Oocyte Complexes: A Refined Approach for Rapid Observation of In Vitro Fertilization
Published on: October 18, 2024
07:45Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation
Published on: July 30, 2020
Related Concept Videos
Oogenesis
Oogenesis
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is known...
Folliculogenesis
Fertilization
Ovarian Cycle