Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Non-disruptive in vitro monitoring of cellular states with cell-free DNA methylation.

Genome biology·2026

Same author

Unmasking metabolic disruptors: The NEMESIS project's quest for Novel Biomarkers, Evidence on Adverse Effects, and Efficient Methodologies.

Open research Europe·2026

Same author

Author Correction: Towards a reference cell atlas of liver diversity over the human lifespan.

Nature reviews. Gastroenterology & hepatology·2025

Same author

Towards a reference cell atlas of liver diversity over the human lifespan.

Nature reviews. Gastroenterology & hepatology·2025

Same author

Wnt signalling maintains self-renewal of human hepatoblasts without blocking their differentiation.

Development (Cambridge, England)·2025

Same author

Transcriptomic and epigenetic mechanisms controlling cholangiocyte transdifferentiation into hepatocytes.

Journal of hepatology·2025

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Published on: June 21, 2012

Activin/nodal signaling and pluripotency.

Zhenzhi Chng¹, Ludovic Vallier, Roger Pedersen

¹Institute of Medical Biology, Singapore, Singapore.

Vitamins and Hormones

|March 1, 2011

Summary

Embryonic stem cells maintain pluripotency, crucial for development. The Activin/Nodal pathway is key to controlling stem cell pluripotency and differentiation for regenerative medicine applications.

Area of Science:

Developmental Biology
Stem Cell Biology
Molecular Signaling

Background:

Pluripotent stem cells, including embryonic stem cells, are vital for mammalian development.
These cells proliferate indefinitely in vitro and can differentiate into all three germ layers.
Understanding stem cell regulation is essential for regenerative medicine.

Purpose of the Study:

To differentiate between various pluripotent cell types.
To explore the molecular mechanisms of Activin/Nodal signaling in pluripotency and differentiation.
To provide knowledge-based strategies for stem cell manipulation.

Main Methods:

Comparative analysis of pluripotent cell types.
Review of current literature on Activin/Nodal signaling pathways.

More Related Videos

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Published on: June 21, 2012

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Molecular mechanism investigation.

Main Results:

Differences among pluripotent cell types were elucidated.
Key roles of Activin/Nodal signaling in maintaining pluripotency were identified.
Insights into differentiation control by Activin/Nodal were discussed.

Conclusions:

Activin/Nodal signaling is a critical regulator of stem cell pluripotency and differentiation.
This pathway offers potential targets for stem cell therapies.
Further research can advance regenerative medicine strategies.