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Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
Published on: September 25, 2014
A multiplex polymerase chain reaction method for rapid pneumococcal serotype determination in childhood empyema
Naouale Maataoui1, Philippe Bidet, Catherine Doit
1Service de Microbiologie, Hôpital Robert-Debré (APHP), 75019 Paris, Université Denis Diderot-Paris 7, France.
Diagnostic Microbiology and Infectious Disease
|March 1, 2011
Abstract:
Nonvaccine serotypes are increasingly responsible for pneumococcal empyema. We developed a one-step polymerase chain reaction for identifying all serotypes covered by the 13-valent conjugate vaccine. Over 1 year, we identified serotypes 19A (n = 6), 1 (n = 2), 3 (n = 1) and 7F/A (n = 2), both in culture-positive and culture-negative pleural fluid from 11 children with empyema.
