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Updated: Jun 4, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
High-throughput screen of protein expression levels induced by cyclooxygenase-2 during influenza a virus infection
1The State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Background:
Detection of proteins productions and the functions during the influenza A virus infection, especially the proteins expression levels in the patients serum are emphasized in the research of host immune response to influenza virus infection. Protein microarray technology provides a high-throughput platform for efficient profiling of protein expression.
Methods:
We investigated the expression levels of 507 immune-related proteins in the 85 serum of patients infected by influenza A virus H3N2 using an antibody array and validated these findings by infecting peripheral blood mononuclear cells (PBMCs) with A/HongKong/498/97 (H3N2) in vitro. Then we used selective inhibitor of proinflammatory factor cyclooxygenase-2 (COX-2) NS-398 to identify those immunomodulatory proteins regulated by the proinflammatory factor.
Results:
In patients' serum, the expression levels of 138 proteins changed >2-fold in response to viral infection, including 102 that were upregulated and 36 that were downregulated. One-hundred six proteins were confirmed in PBMCs infected by H3N2. Of the 106 proteins involved in the immune response to influenza virus infection, 48 were regulated by COX-2.
Conclusions:
Our findings identify the host proteins whose expression levels change in response to influenza virus infection and those involved in the proinflammatory factor COX-2-mediated inflammatory cascade.
