5α-Dihydrotestosterone is a potent androgen in the fathead minnow (Pimephales promelas)

Dihydrotestosterone (DHT) is one of the most physiologically important androgens in many male vertebrates, with the exception of teleost fish, in which 11-ketotestosterone (KT) is generally considered the major circulating male androgen. In the present study, we investigated the effects of KT and DHT on fathead minnow juveniles (Pimephales promelas), with the aim to compare the effects of the two androgens on critical physiological processes, such as somatic growth, male secondary sexual characteristics expression, and gonad maturation. Juvenile fish (60 days post-hatch) were exposed to 20 and 200 ng/L of KT and DHT for 45 days. Exposure to both androgens significantly stimulated somatic growth in both males (20 and 200 ng/L) and females (200 ng/L). Nuptial tubercle formation was induced by both KT and DHT, but only the latter, at 200 ng/L, caused the appearance of dorsal fin spot in 92% of males and 75% of females. Circulating plasma T concentrations showed a sex-specific response; a significant increase was recorded in exposed males and a decrease in females. Both androgens induced a significant advancement of the spermatogenic processes in males at 200 ng/L. In contrast, only DHT caused a severe disruption of ovarian physiology and morphology in females, inducing the development of spermatogenic tissue (intersex). These results show that in fathead minnow juveniles, DHT had in vivo androgenic potency comparable to KT in males, and higher than KT in females, suggesting a potential involvement of DHT in the mediation of fathead minnow androgenic responses.