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Updated: Jun 4, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Chemical constituents of caulis Marsdeniae tenocissimae]
Hui Zhang1, Zhidong Pei, Chunxue Li
1Department of drug, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. syyycs@163.com
Objective:
To study the chemical constitutes of caulis Marsdeniae tenocissimae.
Method:
70% ethanol extracts of caulis M. tenocissimae were isolated and purified by silica gel column chromatography, Sephadex LH-20 chromatography and semi-preparative reversed phase liquid chromatography (RPLC). The compounds were identified on the basis of spectral analysis, including NMR, MS and IR.
Result:
Seven compounds were obtained and identified and their structures were identified as beta-sitosterol (1), condutirol(2), dihydroconduritol(3), betulinic acid(4), lupeol(5), daucosterol(6), 11alpha-O-(2-methylbutyryl)-12beta-O-acetyltenacigeninB(7).
Conclusion:
Compound 4, 5 were isolated from the genus for the first time.
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