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Updated: Jun 4, 2026
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Determination of 5-hydroxy-7-methoxyflavan in Daemonorops draco by HPLC]
Meihong Fu1, Jing Fang, Hongjun Yang
1Institute of Chinses Materia Medica, China Academy of Traditional Chinese Medicine, Beifing 100700, China. fuoo126@sina.com
Objective:
To establish a method for the determination of 5-hydroxy-7-methoxyflavan in Daemrnonorops draco by HPLC.
Method:
Dikma Diamonsil C15 column(4. 6 mm x 150 mm, 5 microm) was used. The mobile phase consisted of (60:40). The temperature of column was 30 degrees C. The flow rate was 1:0 mL x min(-1) and the detection wavelength was at 210 nm.
Result:
The calibration curve was in good linearity with in the range from 0.01 to 0.10 microg (r = 0.9999). The average recovery was 100.1% (n=6).
Conclusion:
The method is simple, rapid, as well as precise and reliable, and can be used for the determination of 5-hydroxy-7-methoxyflavan in D. draco.
