Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

Downstream Processing

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First total synthesis and evaluation of the α-glucosidase inhibitory activity of (±)-methyl dihydromarmesinate, an anti-inflammatory phenylpropanoid from <i>Ficus hirta</i> Vahl., and related benzofurans.

Organic & biomolecular chemistry·2026

Same author

Synthesis and evaluation of analogs of tetracyclic <i>Ganoderma</i> alkaloids as inhibitors of the enzyme α-glucosidase.

Organic & biomolecular chemistry·2025

Same author

The Histidine Kinase VraS Interacts with Vancomycin and Penicillins Through its Membrane-Anchored N-terminal Domain.

Angewandte Chemie (International ed. in English)·2025

Same author

New semisynthetic α-glucosidase inhibitor from a doubly-chemically engineered extract.

Natural products and bioprospecting·2025

Same author

1,3,4-oxadiazoles as inhibitors of the atypical member of the BET family bromodomain factor 3 from <i>Trypanosoma cruzi</i> (<i>Tc</i>BDF3).

Frontiers in microbiology·2024

Same author

Multilevel Dynamic System as Molecular Morning-After Timer.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Published on: August 23, 2024

Chemically engineered extracts: source of bioactive compounds.

I Ayelen Ramallo¹, Mario O Salazar, Luciana Mendez

¹Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Instituto de Química de Rosario, Argentina.

Accounts of Chemical Research

|March 2, 2011

Summary

This summary is machine-generated.

Chemically engineering natural product mixtures creates novel compounds with diverse biological activities. This approach enhances drug discovery by diversifying molecular scaffolds and generating new lead compounds.

More Related Videos

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Related Experiment Videos

Last Updated: Jun 4, 2026

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Published on: August 23, 2024

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

Medicinal Chemistry
Natural Products Chemistry
Drug Discovery

Background:

Natural products are privileged scaffolds in drug discovery due to their evolutionary refinement.
Distinguishing structural properties exist between natural and synthetic compounds, including chiral centers and heteroatom content.
Recurring natural product scaffolds offer unique bioactivity not found in current drugs.

Purpose of the Study:

To prepare libraries of semisynthetic compounds, termed chemically engineered extracts (CEEs), via diversification of natural product mixtures.
To explore the chemical modification of common reactive fragments in natural products to alter extract composition.
To investigate the generation of novel biological activities through chemical diversification.

Main Methods:

Identification of common reactive functional groups in natural products using the Dictionary of Natural Products.
Chemical transformation of natural product mixtures to incorporate nitrogen, sulfur, and bromine.
Isolation and characterization of bioactive compounds from the resulting CEEs.

Main Results:

CEEs exhibited altered composition and biomolecular properties compared to parent natural products.
A semisynthetic β-glucosidase inhibitor was isolated from a CEE derived from reaction with benzenesulfonyl chloride.
An antifungal pyrazole and an acetylcholinesterase inhibitor were identified from CEEs generated using hydrazine and bromination, respectively.

Conclusions:

Chemical diversification of natural product mixtures can generate novel biological activities.
The CEE approach offers a powerful strategy for library preparation in drug discovery.
Judicious experimental design allows for control and potential development of CEEs for research and therapeutic applications.