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PCR01:32

PCR

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Related Experiment Video

Updated: Jun 4, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
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Published on: February 17, 2017

GenomePlex Whole-Genome Amplification.

Nona Arneson, Simon Hughes, Richard Houlston

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    GenomePlex Whole-Genome Amplification (WGA) generates microgram DNA quantities from nanogram samples with minimal bias. This PCR-based method is robust, adaptable to various DNA sources, and ideal for molecular library creation.

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    Area of Science:

    • Molecular Biology
    • Genomics

    Background:

    • Whole-genome amplification (WGA) is crucial for obtaining sufficient DNA for analysis from limited samples.
    • PCR-based WGA methods offer advantages in terms of DNA quality tolerance and source versatility compared to other techniques.

    Purpose of the Study:

    • To describe the GenomePlex WGA protocol for generating microgram quantities of genome-representative DNA.
    • To highlight the nonenzymatic random fragmentation and adaptor ligation approach for DNA amplification.

    Main Methods:

    • Utilizes proprietary GenomePlex WGA technology based on nonenzymatic random DNA fragmentation.
    • Involves converting genomic DNA into a molecular library, adding adaptor sequences, and performing PCR amplification.
    • The protocol is effective with starting DNA amounts as low as 10-100 ng.

    Main Results:

    • Generates microgram quantities of DNA from picogram or nanogram starting material.
    • Achieves significant amplification (several 1000-fold) with minimal representational bias.
    • Demonstrates applicability to DNA from various sources, including fixed and fresh tissues.

    Conclusions:

    • GenomePlex WGA provides a reliable method for substantial DNA yield from minute samples.
    • The technique is suitable for diverse genomic applications requiring high-quality, amplified DNA.
    • Its robustness to DNA quality and source makes it a versatile tool in molecular biology research.