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Updated: Jun 4, 2026

Planarian Ovary Dissection for Ultrastructural Analysis and Antibody Staining
Published on: September 10, 2021
Gene knockdown in planarians using RNA interference
Néstor J Oviedo1, Cindy L Nicolas, Dany S Adams
1Center for Regenerative and Developmental Biology, Forsyth Institute and Developmental Biology Department, Harvard School of Dental Medicine, Boston, MA 02115, USA.
Abstract:
INTRODUCTIONThis protocol describes how to produce gene knockdown in planarians using RNA interference (RNAi). It is a standard technique to evaluate gene function during regeneration and tissue maintenance in planarians. The procedure involves microinjecting double-stranded RNA (dsRNA) synthesized in vitro. Depending on the gene target, this technique can produce robust phenotypes that can be further evaluated by diverse macroscopic or microscopic procedures.
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