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Related Experiment Video

Updated: Jun 4, 2026

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
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Mnemiopsis leidyi Spawning and Embryo Collection.

Kevin Pang1, Mark Q Martindale

  • 1Kewalo Marine Laboratory, University of Hawaii, Honolulu, HI 96813, USA.

CSH Protocols
|March 2, 2011
PubMed
Summary
This summary is machine-generated.

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This study details a protocol for collecting embryos from the marine invertebrate Mnemiopsis leidyi, commonly known as sea walnuts. This method facilitates research on ctenophore evolution and development by enabling controlled spawning and embryo collection.

Area of Science:

  • Marine Biology
  • Developmental Biology
  • Evolutionary Biology

Background:

  • Ctenophores (comb jellies) are crucial for understanding animal evolution due to their unique biology and phylogenetic position.
  • Mnemiopsis leidyi, or sea walnuts, are extensively studied due to their size, reproductive capacity, and invasive potential.

Purpose of the Study:

  • To describe a laboratory protocol for collecting embryos from Mnemiopsis leidyi.
  • To enable researchers to obtain large quantities of embryos for developmental and evolutionary studies.

Main Methods:

  • Maintaining adult Mnemiopsis leidyi in aquaria under controlled conditions.
  • Manipulating light cycles to induce daily spawning at a specific time (e.g., 11:00 a.m.).
  • Collecting spawned eggs for subsequent embryo and larval development studies.

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Related Experiment Videos

Last Updated: Jun 4, 2026

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
09:31

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Published on: January 12, 2015

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10:52

Gamete Collection and In Vitro Fertilization of Astyanax mexicanus

Published on: May 25, 2019

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Main Results:

  • The protocol allows for daily collection of embryos from laboratory-reared Mnemiopsis leidyi.
  • Spawning can be reliably induced by controlling the light regimen, deviating from natural dark-triggered spawning.
  • Larval hatching occurs within 18-24 hours post-spawning.

Conclusions:

  • This standardized protocol facilitates the study of ctenophore reproduction and development.
  • Mnemiopsis leidyi serves as a valuable model organism for research in marine invertebrate biology.
  • Controlled embryo collection supports investigations into the evolution of early animal life.