Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Brain Imaging01:14

Brain Imaging

Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans),  magnetic resonance imaging (MRI),  functional magnetic resonance imaging (fMRI), and Transcranial Magnetic Stimulation (TMS).
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mathematical modeling of intracellular osmolarity and cell volume stabilization: The Donnan effect and ion transport.

The Journal of general physiology·2024
Same author

The physical basis of osmosis.

The Journal of general physiology·2023
Same author

A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life.

PLoS biology·2022
Same author

Myogenic contraction of a somatic muscle powers rhythmic flow of hemolymph through Drosophila antennae and generates brain pulsations.

The Journal of experimental biology·2021
Same author

Improving Management of Limb Injuries in Disasters and Conflicts.

Prehospital and disaster medicine·2019
Same author

Correction: Evolution of our understanding of cell volume regulation by the pump-leak mechanism.

The Journal of general physiology·2019

Related Experiment Video

Updated: Jun 4, 2026

Functional Calcium Imaging in Developing Cortical Networks
16:33

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

Imaging FM Dyes in Brain Slices.

Alan R Kay

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    This study presents a method to track synaptic vesicle activity using FM1-43 fluorescent probes in neuronal systems. A novel cyclodextrin-based wash effectively removes extracellular probe, enhancing visualization of vesicular uptake and release dynamics.

    More Related Videos

    Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
    17:16

    Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

    Published on: December 9, 2010

    Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
    06:43

    Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

    Published on: June 20, 2019

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Functional Calcium Imaging in Developing Cortical Networks
    16:33

    Functional Calcium Imaging in Developing Cortical Networks

    Published on: October 22, 2011

    Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
    17:16

    Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

    Published on: December 9, 2010

    Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
    06:43

    Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

    Published on: June 20, 2019

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biochemistry

    Background:

    • Synaptic vesicle cycling is crucial for neuronal communication.
    • Monitoring vesicle loading and unloading is essential for understanding synaptic function.
    • Existing methods face challenges with extracellular probe interference.

    Purpose of the Study:

    • To describe a generalizable method for monitoring activity-dependent synaptic vesicle dynamics.
    • To address signal interference from extracellular fluorescent probes in intact neuronal systems.
    • To enable clearer visualization of endocytosis and exocytosis in various biological preparations.

    Main Methods:

    • Utilizes styryl pyridinium fluorescent probes (e.g., FM1-43) that partition into lipid membranes.
    • Relies on probe uptake via endocytosis and release during exocytosis.
    • Employs a modified cyclodextrin (Advasep-7) to scavenge and remove extracellular probe.

    Main Results:

    • The method allows for monitoring of synaptic vesicle loading and unloading.
    • Advasep-7 effectively removes extracellular FM1-43, reducing background fluorescence.
    • Enhanced signal-to-noise ratio permits clearer observation of vesicle trafficking events.

    Conclusions:

    • This protocol provides a robust method for studying synaptic vesicle dynamics in diverse neuronal preparations.
    • The use of cyclodextrins significantly improves the sensitivity and accuracy of fluorescent probe-based assays.
    • The technique is applicable to various biological systems beyond the nervous system.